This may be relevant to the human disease, as Hasegawa and collaborators have reported that polymorphism in the C3, C3a receptor, and C5 genes affect susceptibility to bronchial asthma in human beings [28]. Notably, mice sensitized with BtE produced higher amounts of total IgE than those sensitized with OVA, in amounts similar to those observed with immunization with helminth antigens [32]. Mite allergens are considered one of the most potent allergens in house dust. *p < 0.05, **p < 0.01, ***p < 0.001; Tukey's test. Although a mouse model for B. tropicalis extract (Bt E)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. High frequencies of positivity to B. tropicalis antigens in skin prick tests have been described in asthma and rhinitis patients, such as 68.1% in Cuba [7], 91.6% in Venezuela [8], 73.3% in Taiwan [9] and 95.0% in São Paulo, Brazil [10]. Article  On the other hand, a protocol developed by Eum and collaborators [26], using OVA, showed that shortening the duration of the allergic protocol did not affect the immunopathological features of the experimental disease, when it was compared with classical protocols [24]. Concentration-response curves were calculated from the area under the curve, i.e. 072405/Z/03/Z). Int Arch Allergy Immunol 1998,115(2):144–9. These works were carried out using single (A/Sn or BALB/c) mouse strains, and, to the best of our knowledge, no work comparing the allergic response to B. tropicalis antigens in different mouse strains has been done so far. PubMed Central  Eum SY, Hailé S, Lefort J, Huerre M, Vargaftig BB: Eosinophil recruitment into the respiratory epithelium following antigenic challenge in hyper-IgE mice is accompanied by interleukin 5-dependent bronchial hyperresponsiveness. Am J Physiol Lung Cell Mol Physiol 2003,285(1):L32–42. de Brito CA, Fusaro AE, Victor JR, Rigato PO, Goldoni AG, Muniz BP, Duarte AJS, Sato MN, Bruno : CpG-Induced Th1-Type Response in the Downmodulation of Early Development of Allergy and Inhibition of B7 Expression on T Cells of Newborn Mice. Only in A/J and CBA/J mice these total cell counts differed significantly from their saline controls (p < 0.05; Tukey's test; Figure 1A). It is abbreviated Blot by the World Health Organization. Although a mouse model for B. tropicalis extract (BtE)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. Geographical distribution See common geographical background to mites in our Scientific Documents (link to the right). É sabidamente causador de alergias e crises de asma e rinite alérgica em humanos. Лаймската болест (Morbus laimi, Лаймска борелиоза, борелиозис бургдорфери или само борелиоза) е инфекциозно заболяване, причинявано от поне четири различни вида бактерии представители на рода Борелия. Article  MR contributed in planning the experiments and reviewing the manuscript. In our model, eosinophils were the preponderant cells in the airways, however similar numbers of neutrophils and eosinophils were found in airways when the animals were sensitized without alum (data not shown). The cell pellets were resuspended in PBS and lysed by three successive freezing and/thawing procedures, and then assayed for peroxidase activity in 96-well microassay plates, in duplicates, using 6.6 mM H2O2 and 1.5 mM orthophenylenodiamine (Merck, Whitehouse Station, NJ, USA). (F and G) representative lung sections stained with hematoxylin and eosin of a saline-injected animal (F) and a Bt-sensitized animal (G). BtE-sensitized animals had more total serum IgE as well as higher titers of anti-BtE IgE antibodies than the saline control group (Figure 5D and 5E; p < 0.05; Student's t test and Mann-Whitney's test, respectively). Altogether, we concluded that the short-term experimental model of BtE-induced asthma is reproducible in different mouse strains, although the A/J mice are the best responders, and small quantities of BtE may be used to sensitize this mouse strain. The washings were carried out until no food residues could be seen under microscopy. P > 0.05 is not represented. BtE-immunized mice of the most responsive strain (A/J strain) were then assessed for the presence of intra-bronchial mucous, airway hyperresponsiveness (AHR) to methacholine challenge and inflammatory cell infiltration in lung tissue. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE. This study included the comparison of the allergic responses elicited by BtE with those elicited by ovalbumin in mice of the strain that responded better to BtE sensitization. P > 0.05 is not represented. Health issues Allergies . There is evidence that allergens from B. tropicalis are distinct from, and bear only low to moderate cross-reactivity to allergens from Dermatophagoides sp. The authors declare that they have no competing interests. This work had two objectives. Acest acarian are dimensiuni foarte mici și este vizibil doar pe un fundal întunecat la o lumină normală. A significantly larger number of cells was found in the BALF of the mice sensitized with low-dose of BtE than in the BALF of the saline control group (Figure 5A; p < 0.01; Student's t test). Tissues sections of 5-μm were then stained with periodic acid-Schiff (PAS) for the evaluation of mucus production. The mites were lysed in 0.15 M phosphate-buffered saline, pH 7.4 (PBS), in a blender (Waring Commercial, Torrington, Connecticut, USA). Additionally, there were high levels of IL-4 and IL-13 in BALF and increased levels of specific IgE in the sera. Int Arch Allergy Immunol 2001,126(4):286–93. IFN-γ and IL-10 concentrations in the BALF from BtE-sensitized or saline-treated mice of all tested mouse strains were low, and no statistically significant differences were found among the studied groups and their negative controls (data not shown). tropicalisextract (Bt E) on the development of experimental respiratory allergy in A/J mice. However, results obtained in murine experimental models of respiratory allergy that use OVA as antigen differ from those obtained in experimental models using mite allergens. Entry date: September 24, 2002 12:00 +1GMT: Last update: July 24, 2019 20:03 +1GMT: Allergome Code: 152 En climas quentes, atópase a Blomia tropicalis. Data from A, B, and C are representative of three experiments, and from D of two experiments. Prof. Lineu Prestes, 1730, Cidade Universitária, Butantã, CEP 05508-900, Brazil, Momtchilo Russo, Eliane Gomes & Renato Barboza, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Rua Waldemar Falcão, 121, Brotas, Salvador, Bahia, CEP 40296710, Brazil, Virgínia MG Silva, Thayna Meirelles, Pablo RS Oliveira & Lain Pontes-de-Carvalho, You can also search for this author in This article is published under license to BioMed Central Ltd. Int Arch Allergy Immunol 2003,132(4):346–54. Our mission is to enable our customers to make the world healthier, cleaner, and safer. EGFR and STAT6 signaling were not investigated in the present study, but we found increased IL-13 levels, in relation to saline-treated controls, in BtE-sensitized A/J mice. Lowry OH, Rosenbrough NJ, Faar AL, Randall RJ: Protein measurement with the folin phenol reagent. This data is representative of three independent experiments. The relevance and reproducibility of experimental animal models of allergy depends on the genetic background of the animal, the molecular composition of the allergen and the experimental protocol. After the BALF collection, the lungs were perfused, via the heart right ventricle, to remove residual blood, immersed in 10% phosphate-buffered formalin for 24 h, followed by 70% ethanol, and embedded in paraffin. Finally, they had intense AHR. As a global leader in allergy and autoimmune disease diagnostics, we are committed to providing accurate, clinically relevant testing solutions to aid in the proper disease diagnosis and patient management. (B) Differential leukocyte numbers in BALF. Blomia tropicalis allergens are the most important mite allergens in tropical regions. Manage cookies/Do not sell my data we use in the preference centre. Retrieved from " https://wiki.pestinfo.org/w/index.php?title=Blomia_tropicalis&oldid=368042 " In B and D, columns represent the mean result of 5 (B) or 8 (D) animals; the vertical bars represent the standard deviation of the means. http://creativecommons.org/licenses/by/2.0. Google Scholar. LPC participated in planning the experiments and reviewing the manuscript. … Springer Nature. J Immunol Methods 1985,83(2):209–15. Mota I, Wong D: Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization. The described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. PubMed  For instance, antibodies from allergic patients against the main B. tropicalis allergens (proteins of 14.3 and 27.3 kDa) do not inhibit the binding of anti-D. pteronyssinus antibodies to D. pteronyssinus antigens [4, 9, 11]. EPO activity in BALF was also higher in BtE-sensitized than in OVA-sensitized and control mice (p < 0.05 and p < 0.001, respectively; Tukey's test; Figure 2C). BtE-sensitized and challenged BALB/c or C57Bl/6 mice produced low amounts of IL-4, which were similar to those produced by their saline-treated control groups (p > 0.05, Tukey's test; Figure 1E). Animals sensitized and challenged with BtE showed higher levels of total cells and eosinophils in the BALF than control mice (p < 0.001, Figure 2A, and p < 0.01, Figure 2B; Tukey's test). In Europe, detectable Der p 1 or Der f 1 allergen was found in 68% of surveyed homes. Clin Exp Allergy 2002, 32:602–611. The mucus index was higher in mice sensitized with OVA than in mice sensitized with BtE or in the mice of the saline-treated control group (Figure 3A; p < 0.001 and p < 0.01, respectively; ANOVA and Tukey's test). This model may constitute a better approximation to a natural allergenic sensitization, in which allergic individuals tend to be exposed to low allergen doses, independently of the entry route, than the so far published experimental murine models, that use higher BtE doses [[14–16] and [35]]. Tsai JJ, Wu HH, Shen HD, Hsu EL, Wang SR: Sensitization to Blomia tropicalis among asthmatic patients in Taiwan. (F) IL-13 concentration in BALF. (G) Anti-B. Blomia tropicalis, a Storage mite, was earlier found predominantly in agricultural environments but is now being recognised as an important contributor to the allergen content in house dust in indoor urban dwellings. Cohn L: Mucus in chronic airway diseases: sorting out the sticky details. (B) Differential cell count in the BALF. No differences in numbers of macrophage, lymphocyte and neutrophils in the BALF were observed among the mice of all four strains (p > 0.05, ANOVA; data not shown). Strath M, Warren DJ, Sanderson CJ: Detection of eosinophils using an eosinophil peroxidase assay. Brewer JP, Kisselgof AB, Martin TR: Genetic variability in pulmonary physiological, cellular, and antibody responses to antigen in mice. However, in humans, most cases of asthma are due to aeroallergens, and OVA-induced asthma is far from being a common event. Correspondence to (E) IgE anti-Bt antibody serum titers as determined by passive cutaneous anaphylaxis (PCA). After the observation that A/J was the best mouse strain for BtE-induced asthma, we immunized these mice with a low-dose (10 μg per injection) of BtE instead of the 100 μg dose per injection used in the previous experiments. On the other hand, OVA sensitization led to higher mucus production, and serum levels of specific IgE, IgG1 and IgG2a than BtE sensitization. PubMed  Espesye sa uhong sakop sa division nga Ascomycota ang Candida tropicalis.Una ning gihulagway ni Aldo Castellani, ug nga gihatagan sa eksakto nga ngalan ni Christine Marie Berkhout ni adtong 1923. Smith H: Animal models of asthma. Eur J Immunol 2007, 37:1170–1173. Cheong N, Siew CS, Ramos JDA, Kuo IC, Prasanna K, Lee BW, Chua KY. Their body parts and fecal excreta are initially 10 to 50 mm in diameter but break down into smaller fragments that become airborne when dust is disturbed. tropicalis IgE antibodies. tropicalis IgE antibody levels as titrated by passive cutaneous anaphilaxis (PCA). The circunference area of the bronchi in the PAS-stained area was electronically measured and the mucus index was determined by the following formula: Mucus index = (PAS-stained area/bronquial cross-section area) × 100. El măsoară 420 micrometri în lungime și 250-320 microni în lățime. In A and C, each symbol corresponds to the result obtained from an individual animal. Finally, immunization with a relatively low BtE dose (10 μg per subcutaneous injection per mouse) was able to sensitize A/J mice, which were the best responders to high-dose BtE immunization, for the development of allergy-associated immune and lung inflammatory responses. ) representative lung sections stained with periodic acid-Schiff ( PAS ) for the development of allergic diseases [ ]. ) levels of eosinophil peroxidase ( EPO ) activity in BALF development of a protocol... Conducted the majority of the experiments and wrote the first manuscript draft, Siew CS Ramos. Lepidoglyphus destructor mostly present among agriculture population used in different mouse strains in... Asthmatic response [ 17, 18 ] BALF and increased levels of IL-4 and IL-13 in BALF, B and... Experiments, and the cell pellet resuspended in PBS-BSA junto ao estrado e na face )! 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blomia tropicalis wikipedia

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